Rapid and transient rise in diacylglycerol concentration in Daudi cells exposed to interferon.

Rapid and transient rise in diacylglycerol concentration in Daudi cells exposed to interferon.

Human beta-interferon stimulates a 4-fold increase in the concentration of diacyglycerol and a 2-fold increase in the concentration of inositol monophosphate in Daudi (human B-lymphoblastoid) cells within 30 sec of exposure of the cells to interferon. The increase in diacylglycerol and in inositol monophosphate is transient and the concentrations of these compounds decrease to basal levels with...

Interferon-Resistant Daudi Cells

Interferon - a Down - regulates Insulin Receptors in Lymphoblastoid ( Daudi ) Cells

The Daudi line of human lymphoblastoid cells requires insulin and transferrin for growth in serumfree medium and is highly sensitive to the inhibitory effect of human leukocyte interferon (IFN-a) on cell proliferation. A variant subline of Daudi cells, which is resistant to the antiproliferative action of IFN-a, also has been grown in serum-free medium containing insulin and transferrin. The pr...

Interferon-induced gene expression in wild-type and interferon-resistant human lymphoblastoid (Daudi) cells.

Interferon-induced gene expression was analyzed in wild-type and interferon-resistant Daudi cells. Two classes of alpha-interferon-induced mRNAs and proteins were observed: those that were similarly induced in both types of cell and those that were induced only in the wild-type cells. Furthermore, the level of c-myc mRNA decreased in the wild-type but not in the resistant cells. This differenti...

Rapid variation in kidney histology in spotted scat Scatophagus argus on exposed to abrupt salinity changes

In order to study the role of the kidney in fish osmoregulation, freshwater acclimatized juvenile spottedscat (Scatophagus argus L.) were subjected to four different salinities and observed for histomorphometricchanges of the kidney at 1, 2, 10 and 15 days post transfer time points. The overall morphological changesdisplayed by fish kidney included significant decrease in the density of collect...